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faouzi laachari lipase activity

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faouzi laachari lipase activity

(PDF) Biochemical study of lipase from Bacillus subtilis

Faouzi Laachari 1, Fatimazahra El Bergadi 1,2, Wifak Bahafid 1,2, Adel Sayari 3, Soumya Maximum lipase activity was found in the presence of Ca ions Olive oil and Tween 80 induced lipaseFaouzi Laachari Lipase Activity Isolation Purification And Characterization Of A Cold Aspergillus nidulans WG312 strain secreted lipase activity when cultured in liquid media with olive oil as carbon source Highest lipase productivity was found when the mycelium was grown at 30Faouzi Laachari Lipase Activity soby

Biochemical study of lipases from Bacillus subtilis

Faouzi Laachari1, Fatimazahra El Bergadi1,2, lipase activities Gene which encodes the 16S ribosomal RNA for bacterial species was the activity of lipase was examined within the pHFaouzi Laachari, Soumya Elabed, Adel Sayari, Iraqui Mohammed, El Hassan Harchali, Saad Koraichi Ibnsouda Biochemical characterization of a thermoactive and thermostable lipase from a newly olatedFaouzi LAACHARI | Faculté des Sciences et Techniques Fès

Faouzi Laachari Lipase Activity nautiflexit

Faouzi Laachari, Fatimazahra El Bergadi, Saad Kor Ibnsouda Laboratoire de Biotechnologie Microbienne Faculté des Sciences et Techniques de Fès Saïs Université Sidi Mohamed Ben Abdellah BP 2202 – Route d’Imouzzer FèsMaroc Email: [ protected] Abstract Aspergillus flavus lipase (AFL) was found as a true lipaseSimilarly, Faouzi et al, (2015) reported that lipase produced by B pumilus exhibited optimum activity at 45 o C The previous study of Lima et al, (2012) recorded that the optimum lipaseBiochemical Characterization of a New Thermostable Lipase

Biochemical characterization of a thermoactive and

efficiently than the short ones A specific activity of 1800 U/mg was measured on tributyrin or olive oil emulsion as substrate This newly isolated lipase can be considered as a good candidature for industrial and biotechnological applications Key words: Trichosporon coremiiforme, lipase, purification, thermoactive INTRODUCTIONThe main objective of this work was to identify novel lipases of industrial interest In this paper, Aspergillus flavus lipase (AFL) was isolated from the traditional tannery of Fez city in Morocco; it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate (NaDC) Bile salts showed no inhibitory effect on the lipolytic activity, whereasAfrican Journal of Biochemistry Research higher

faouzi laachari lipase activity saxofoonschoolijburgnl

According to the results, we noticed that the lipase activity performance measured by the modified protocol was much improved, scoring a maximum of 14 Université Sidi Mohamed Ben Abdellah Faculté des Sciences Purification and Characterization of a Novel Thermostable Lipase from Aspergillus Flavus Faouzi Laachari Abstractfaouzi laachari lipase activity Purifiion and Biochemical Characterization of an Acid Control experiments were performed under the same experimental conditions, with dromedary and turkey pancreatic lipases and showed a positive interfacial activation phenomenon Sodium deoxycholate (NaDC) has an inhibitory effect on the lipase activityfaouzi laachari lipase activity Simulation assurance auto

African Journal of Biochemistry Research higher

The main objective of this work was to identify novel lipases of industrial interest In this paper, Aspergillus flavus lipase (AFL) was isolated from the traditional tannery of Fez city in Morocco; it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate (NaDC) Bile salts showed no inhibitory effect on the lipolytic activity, whereasNonstop demand for greatly thermostable and thermoactive active lipase encourages the research for the new enzyme sources In this study, a strain of Trichosporon coremiiforme was isolated from the traditional tannery in the city of Fez in Morocco, lipase production and their lipolytic activity was studied Pure T coremiiforme lipase (TCL) was obtained after ammonium sulfate fractionation, GBiochemical characterization of a thermoactive and

Scilit | Article Higher tolerance of a novel lipase from

The main objective of this work was to identify novel lipases of industrial interest In this paper, Aspergillus flavus lipase (AFL) was isolated from the traditional tannery of Fez city in Morocco; it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate (NaDC) Bile salts showed no inhibitory effect on the lipolytic activity, whereasHigher tolerance of a novel lipase from Aspergillus flavus to the presence of free fatty acids at lipid/water interface Faouzi Laachari1*, Fatimazahra El Bergadi1, Moulay Sadiki1, Adel Sayari3, Wifak Bahafid1, Soumya Elabed2, Iraqui Mohammed1 and Saad Koraichi Ibnsouda1 1Laboratoire de Biotechnologie Microbienne Université Sidi Mohamed BenHigher tolerance of a novel lipase from Aspergillus flavus

International Journal of Research (IJR)

Faouzi Laachari, Fatimazahra El Bergadi, Saad Koraichi Ibnsouda Laboratoire de Biotechnologie Microbienne Faculté des Sciences et Techniques de Fès Saïs Université Sidi Mohamed Ben Abdellah BP 2202 – Route d’Imouzzer FèsMaroc Email: Abstract Aspergillus flavus lipase (AFL) was found as a true lipaseLipase activity determination The lipase activity was measured titrimetrically at pH 82 and 45°C with a pHstat under standard conditions using tributyrin (025 ml) in 30 ml of 25 mM Tris–HCl pH 82, 2 mM CaCl 2, 2 mM NaDC or olive oil emulsion (10 ml in Turk J Biochem 2015; 40(1):814 9 LaachariBiochemical characterization of a new thermostable lipase

Biochemical characterization of a new thermostable lipase

Mar 01, 2015· The activity of lipase was examined within the pH range of 60100 and the effect of pH on lipase stability was determined by incubating the lipase fraction in various buffer solutions ranging from 30 to 100 for 24 h at room temperature Results: The results showed that Bacillus pumilus is a strain that produce noninducible lipaseOct 18, 2013· The content of libraries in Fez is an important historical testimony and a treasure of knowledge of several civilizations Unfortunately, this cultural heritage has suffered deterioration caused by many factors, including microbial deterioration specifically by cellulolytic fungi The aim of our study was the identification and characterization of the microflora that damage historicalCellulolytic potential and filter paper activity of fungi

Purification and Characterization of a Novel Thermostable

Faouzi Laachari Abstract This newly isolated lipase can be considered as a good candidature in synthesis reactions such as esterification, transesterification and interesterification which are used in industrial and biotechnological applicationsLaachari F, El Bergad F, Sadiki M, et al Higher tolerance of a novel lipase from Aspergillus flavus to the presence of free fatty acids at lipid/water interface Afr J Biochem 2015;Lipases: Sources, Production, Purification, and

Cellulolytic potential and filter paper activity of fungi

A total of 31 filamentous fungi were isolated from deteriorated paper of ancient books Nine of these isolates were screened positive for the ability to degrade carboxymethylcellulose (CMC) The ability of the nine isolates to produce filter paper activity (FPUase) in liquid media as well as the effect of temperature and pH were also studiedThe purified enzyme exhibited optimum activity at temperature 60°C, pH 60, Km of 183 mM l and the Vmax of 100 mM l min 1 11 EDTAK almost completely inhibited the lipase activity The enzyme had property to tolerate a wide range of organic solvents which makeLipase from Bacillus pumilus RK31: Production

faouzi laachari lipase activity saxofoonschoolijburgnl

Purification and Characterization of a Novel Thermostable

Faouzi Laachari Abstract This newly isolated lipase can be considered as a good candidature in synthesis reactions such as esterification, transesterification and interesterification which are used in industrial and biotechnological applicationsMar 01, 2015· The activity of lipase was examined within the pH range of 60100 and the effect of pH on lipase stability was determined by incubating the lipase fraction in various buffer solutions ranging from 30 to 100 for 24 h at room temperature Results: The results showed that Bacillus pumilus is a strain that produce noninducible lipaseBiochemical characterization of a new thermostable lipase

Lipases: Sources, Production, Purification, and

Laachari F, El Bergad F, Sadiki M, et al Higher tolerance of a novel lipase from Aspergillus flavus to the presence of free fatty acids at lipid/water interface Afr J Biochem 2015;A total of 31 filamentous fungi were isolated from deteriorated paper of ancient books Nine of these isolates were screened positive for the ability to degrade carboxymethylcellulose (CMC) The ability of the nine isolates to produce filter paper activity (FPUase) in liquid media as well as the effect of temperature and pH were also studiedCellulolytic potential and filter paper activity of fungi

Lipase from Bacillus pumilus RK31: Production

The purified enzyme exhibited optimum activity at temperature 60°C, pH 60, Km of 183 mM l and the Vmax of 100 mM l min 1 11 EDTAK almost completely inhibited the lipase activity The enzyme had property to tolerate a wide range of organic solvents which makeOct 18, 2013· The content of libraries in Fez is an important historical testimony and a treasure of knowledge of several civilizations Unfortunately, this cultural heritage has suffered deterioration caused by many factors, including microbial deterioration specifically by cellulolytic fungi The aim of our study was the identification and characterization of the microflora that damage historicalCellulolytic potential and filter paper activity of fungi

Bacterial lipases: A review on purification and

Jan 01, 2018· Lipase immobilized on celite could retain 90% lipase activity after eight cycles Transesterification of oil using the immobilized lipase obtained from Botryococcus sp resulted in 80% yield of fatty acid methyl esters which had good properties for use as biodiesel ( Sivaramakrishnan and Incharoensakdi, 2016 )Application Form For Crushing Plant Part B Application Form Bathnes When to use this form This regime is known as environmental permitting Use this form if you are applying for a permit to Bath and North East Somerset Council to operate a mobile crushing and screening plant Which parts of the form to fill in You should fill in as much of this form as possible Readrtgs neft sbgrpt application form

Isolation, Purification, and Characterization of a Cold

Aspergillus nidulans WG312 strain secreted lipase activity when cultured in liquid media with olive oil as carbon source Highest lipase productivity was found when the mycelium was grown at 30 °C in a rich medium The new enzyme was purified to homogeneity from the extracellular culture of A nidulans by phenylSepharose chromatography and affinity binding on linolenic acid−agarosesyndicaliste activity in coal mining jerada city ellulnl Gambar Coal Mining Crusher Activity Indonesia archedyl gambar animasi coal crusher egypt KAMY China gambar stone crusher plant herbalpharma gambar stone crusher plant macam gambar stone crusher gambar stone crusher plant,macam macam gambar stone crusher plant Mining is thesyndicaliste activity in coal mining jerada city

Copper Concentrate Plant In Saudi Arabia

Jun 27, 2013 Jabal Sayid MineSite InfoMine 23 Mar 2012 Mining information for theJabal Sayid mine in Saudi Arabia The Jabal Sayid coppergoldsilver project is located in Saudi Arabia, 350 km The process plant would employ the flotation method and would produce 30 t tipper trucks, which are the same trucks used as the prime mover for the road trainsLowers the noise by 12 dBA A reduction of 10 dBA will sound half as loud Saw cutting of pavers and pavement slabs A cutting station should be established with the saw enclosed in an acoustic enclosureNoise Reduction In Vibratory Screen